Knockdown:Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon-bone healing
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6-1; American Type Culture Collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1×10 6 cells/ml) were seeded in six-well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% ( ).A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the..
Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon‑bone healing.
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6‐1; American Type culture collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1x106 cells/ml) were seeded in six‐well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% (17).A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells..
Expressing:Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon-bone healing
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6-1; American Type Culture Collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1×10 6 cells/ml) were seeded in six-well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% ( ).A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the..
Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon‑bone healing.
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6‐1; American Type culture collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1x106 cells/ml) were seeded in six‐well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% (17).A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells..
Immunofluorescence:Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon-bone healing
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6-1; American Type Culture Collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1×10 6 cells/ml) were seeded in six-well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% ( ).A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the..
Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon‑bone healing.
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6‐1; American Type culture collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1x106 cells/ml) were seeded in six‐well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% (17).A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells..
Staining:Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon-bone healing
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6-1; American Type Culture Collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1×10 6 cells/ml) were seeded in six-well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% ( ).A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the..
Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon‑bone healing.
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6‐1; American Type culture collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1x106 cells/ml) were seeded in six‐well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% (17).A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells..
Flow Cytometry:Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon-bone healing
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6-1; American Type Culture Collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1×10 6 cells/ml) were seeded in six-well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% ( ).A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the..
Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon‑bone healing.
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6‐1; American Type culture collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1x106 cells/ml) were seeded in six‐well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% (17).A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells..
Co-Culture Assay:Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon-bone healing
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6-1; American Type Culture Collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1×10 6 cells/ml) were seeded in six-well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% ( ).A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the..
Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon‑bone healing.
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6‐1; American Type culture collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1x106 cells/ml) were seeded in six‐well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% (17).A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells..
Quantitative RT-PCR:Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon-bone healing
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6-1; American Type Culture Collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1×10 6 cells/ml) were seeded in six-well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% ( ).A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the..
Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon‑bone healing.
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6‐1; American Type culture collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1x106 cells/ml) were seeded in six‐well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% (17).A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells..
Incubation:Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon-bone healing
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6-1; American Type Culture Collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1×10 6 cells/ml) were seeded in six-well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% ( ).A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the..
Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon‑bone healing.
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6‐1; American Type culture collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1x106 cells/ml) were seeded in six‐well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% (17).A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells..
Western Blot:Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon-bone healing
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6-1; American Type Culture Collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1×10 6 cells/ml) were seeded in six-well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% ( ).A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the..
Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon‑bone healing.
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6‐1; American Type culture collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1x106 cells/ml) were seeded in six‐well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% (17).A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells..
Knock-Out:Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon-bone healing
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6-1; American Type Culture Collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1×10 6 cells/ml) were seeded in six-well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% ( ).A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the..
Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon‑bone healing.
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6‐1; American Type culture collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1x106 cells/ml) were seeded in six‐well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% (17).A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells..
Labeling:Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon-bone healing
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6-1; American Type Culture Collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1×10 6 cells/ml) were seeded in six-well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% ( ).A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the..
Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon‑bone healing.
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6‐1; American Type culture collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1x106 cells/ml) were seeded in six‐well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% (17).A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells..
Cell Culture:Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon-bone healing
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6-1; American Type Culture Collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1×10 6 cells/ml) were seeded in six-well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% ( ).A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the..
Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon‑bone healing.
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6‐1; American Type culture collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1x106 cells/ml) were seeded in six‐well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% (17).A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells..
Fluorescence:Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon-bone healing
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6-1; American Type Culture Collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1×10 6 cells/ml) were seeded in six-well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% ( ).A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the..
Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon‑bone healing.
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6‐1; American Type culture collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1x106 cells/ml) were seeded in six‐well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% (17).A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells..
Microscopy:Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon-bone healing
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6-1; American Type Culture Collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1×10 6 cells/ml) were seeded in six-well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% ( ).A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the..
Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon‑bone healing.
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6‐1; American Type culture collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1x106 cells/ml) were seeded in six‐well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% (17).A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells..
Double Staining:Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon-bone healing
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6-1; American Type Culture Collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1×10 6 cells/ml) were seeded in six-well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% ( ).A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the..
Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon‑bone healing.
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6‐1; American Type culture collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1x106 cells/ml) were seeded in six‐well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% (17).A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells..
Negative Control:Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon-bone healing
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6-1; American Type Culture Collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1×10 6 cells/ml) were seeded in six-well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% ( ).A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the..
Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon‑bone healing.
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6‐1; American Type culture collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1x106 cells/ml) were seeded in six‐well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% (17).A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells..
Binding Assay:Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon-bone healing
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6-1; American Type Culture Collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1×10 6 cells/ml) were seeded in six-well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% ( ).A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the..
Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon‑bone healing.
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6‐1; American Type culture collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1x106 cells/ml) were seeded in six‐well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% (17).A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells..
Derivative Assay:Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon-bone healing
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6-1; American Type Culture Collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1×10 6 cells/ml) were seeded in six-well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% ( ).A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the..
Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon‑bone healing.
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6‐1; American Type culture collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1x106 cells/ml) were seeded in six‐well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% (17).A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells..
Reverse Transcription:Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon-bone healing
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6-1; American Type Culture Collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1×10 6 cells/ml) were seeded in six-well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% ( ).A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the..
Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon‑bone healing.
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6‐1; American Type culture collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1x106 cells/ml) were seeded in six‐well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% (17).A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells..
Real-time Polymerase Chain Reaction:Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon-bone healing
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT-qPCR and western blotting (as described below), the cells were seeded at a density of 5×10 5 cells/well in six-well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6-1; American Type Culture Collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1×10 6 cells/ml) were seeded in six-well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% ( ).A 254 nm UV light was used with an energy dose of 200 mJ/cm 2 for 15-20 min. Fresh RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the..
Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon‑bone healing.
Article Snippet: After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.After verifying the Zeb1 knockdown efficiency by RT‐qPCR and western blotting (as described below), the cells were seeded at a density of 5x105 cells/well in six‐well plates for subsequent experiments.. The human lymphocyte line Jurkat (clone E6‐1; American Type culture collection) was selected as the apoptotic cell model. Logarithmic growth phase Jurkat cells (1x106 cells/ml) were seeded in six‐well plates and exposed to ultraviolet (UV) light under uncovered conditions.. A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells were further cultured for 6 h, with an apoptosis rate of ~85% (17).A 254 nm UV light was used with an energy dose of 200 mJ/cm2 for 15‐20 min. Fresh RPMI‐1640 medium (Gibco; Thermo Fisher Scientific, Inc.) [supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.)] was then added and the cells..
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